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| Figure 1. Effect of incubation time on methane produced per unit substrate DM solubilized with potassium nitrate or urea as the NPN source, and molasses and foliages of Paper mulberry and Muntingia as the substrate |
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Reducing Greenhouse Gas Emissions from Livestock and Soils |
The aim of this study was to evaluate the effect of potassium nitrate in reducing methane production in an in vitro incubation with molasses and paper mulberry or muntingia foliages as the substrate. The treatments arranged in a 2*2 factorial with four replications were: potassium nitrate (6% of diet DM basis) or urea (1.83% of diet DM basis); and paper mulberry or muntingia foliage. The quantity of substrate was 12 g to which were added 240 ml rumen fluid (from a buffalo in the slaughter house ) and 960 ml of buffer solution. The incubation was for 24 h with measurements of total gas and percent methane at intervals of 6, 12, 18 and 24 h, and determination of residual unfermented substrate at the end of 24 h.
Gas production, percent methane in the gas and methane produced per unit DM solubilized, at each incubation interval,. were lower with potassium nitrate than with urea as NPN source (Figure 1). The fermentability of the substrate was higher for Paper mulberry than for Muntingia as the forage source but there were no differences between the two forages in methane produced per unit substrate DM solubilized.
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| Figure 1. Effect of incubation time on methane produced per unit substrate DM solubilized with potassium nitrate or urea as the NPN source, and molasses and foliages of Paper mulberry and Muntingia as the substrate |