MEKARN Workshop 2008: Organic rabbit production from forages |
Two experiments were conducted at the experimental farm of Cantho University. In Experiment 1, sixty crossbred rabbits (local x improved breed) at 50 days of age, arranged in a Complete Randomized Design with 5 treatments and 3 replications, were offered levels of Mucuna pruriens forage corresponding to 0, 20, 40, 60 and 80% (DM basis) of the observed intake of para grass on the control (no Mucuna) treatment. Para grass was fed ad libitum and supplements of soybean waste and commercial concentrate were given at levels of 200 and 15 g/day, respectively, in all treatments. In Experiment 2, the experimental design was similar to that of the feeding trial; however, 12-week old rabbits were used. The animals had one week for adaptation and 6 days for recording quantities and taking samples of feces and urine
DM and crude protein (CP) intake increased with increasing ratio of Mucuna to Para grass in the forage fraction of the diet. Growth rate increased with increasing ratio of Mucuna as replacement for Para grass until a substitution level of about 0.50 after which it appeared to decrease. Feed conversion became worse as the proportion of Mucuna increased beyond 0.2. The ratio of Mucuna to Para grass did not affect the apparent digestibility coefficients of DM, OM and CP; however, the coefficients for the NDF and ADF fractions increased up to the 0.50 ratio of Mucuna followed by a decline at the higher level. Intake and retention of N increased with increasing ratios of Mucuna in the forage fraction of the diet.
Introduction
In recent years, rabbit production based on low cost forages has increased considerably in Vietnam in order to meet the increasing demand for human food. However, studies on forage feeding and diet digestibility in growing rabbits are still limited. Mucuna pruriens (MP) is widely grown in the tropics mainly because of the medicinal properties that appear to be found in all fractions of the plant (Sridhar and Bhat 2007). These authors reviewed the use of seeds and leaves of Mucuna as animal feed but rabbits were not mentioned. Mucuna is widely available in the Mekong Delta. The foliage has a higher crude protein content and less fibre than Para grass (Brachiaria mutica). Thus the combination between Mucuna and para grasss in diets for growing rabbits could be efficient in terms of increased nutrient intake. This study aimed to determine the optimum level of Mucuna pruriens as a replacement for Para grass in the diets of growing rabbits.
The experiment was conducted at the experimental farm of Cantho University. Sixty crossbred rabbits (local x improved breed) at 50 days of age (average live weight of 615g) were arranged in a Complete Randomized Design with 5 treatments and 3 replications. Four rabbits (balanced for sex) in one cage was the experimental unit. The treatments were offer levels of Mucuna pruriens corresponding to 0, 20, 40, 60 and 80% (DM basis) (MP0, MP20, MP40, MP60 and MP80) of the observed intake of para grass on the control (MP0) treatment. Para grass was fed ad libitum. Supplements of soybean waste and commercial concentrate were given at levels of 200 and 15 g/day, respectively, in all treatments.
The experimental design was similar to that of the feeding trial; however, 12-week old rabbits were used. The animals had one week for adaptation and 6 days for recording quantities and taking samples of feces and urine.
Para grass and Mucuna pruriens were collected daily in the areas surrounding Cantho city. These feeds were given in fresh form and were offered three times a day at 7:00h, 15:00h and 19:00h. Quantities offered and refusals were recorded daily for each forage. Fresh water was freely available. The rabbits were vaccinated to prevent hemorrhagic and parasite diseases.
Feeds and refusals were analysed for DM, organic matter (OM), crude protein (CP) and ash according to AOAC (1990) procedures. NDF and ADF were determined following procedures of Van Soest et al (1991). Rabbits were weighed weekly.
The data from both experiments were analyzed using the
General Linear Model option in the ANOVA of Minitab (Minitab 2000).
Mucuna pruriens was considerably higher in crude protein, lower in NDF and with similar levels of ADF, compared with para grass (Table 1). Soybean waste and the concentrate had higher levels of crude protein than the forages.
Table 1: Chemical composition of feeds used in Experiment 1 (% in DM, except for DM which is on fresh basis) |
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|
DM |
OM |
CP |
NDF |
ADF |
Ash |
Mucuna pruriens |
19.5 |
92.1 |
14.8 |
47.4 |
38.3 |
7.90 |
Para grass |
18.9 |
88.8 |
9.40 |
71.8 |
35.8 |
11.2 |
Soya waste |
11.1 |
93.2 |
21.7 |
47.9 |
33.4 |
6.80 |
Concentrate |
87.0 |
91.1 |
20.0 |
23.6 |
8.06 |
8.90 |
DM: dry matter, OM: organic matter, CP: crude protein, NDF: neutral detergent fiber, ADF: acid detergent fibre |
Overall intakes of DM, OM, CP and ADF increased with increasing offer level of Mucuna (Table 1). NDF intakes were similar on all treatments. The planned ratios of Mucuna and Para grass were achieved for offer levels up to 40% replacement (M40) of Para grass but at the highest levels the observed replacement values of Mucuna were some 20% lower than planned (Figure 1; Table 3).
Table 2. Daily intakes of forages and proximate constituents in Experiment 1 (g/rabbit/day) |
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|
MP0 |
MP20 |
MP40 |
MP60 |
MP80 |
SEM/Prob. |
Para grass |
46.1a |
38.8b |
32.1bc |
32.1bc |
26.6c |
1.46/0.001 |
Mucuna pruriens |
0.00a |
9.90b |
19.8c |
29.6d |
39.1e |
0.59/0.001 |
DM |
78.3a |
81.3a |
84.5a |
94.3b |
98.1b |
1.40/0.001 |
OM |
71.7a |
74.5ab |
77.6b |
86.6c |
90.2c |
1.24/0.001 |
CP |
12.1a |
12.7b |
13.5c |
14.9d |
15.8e |
0.11/0.001 |
NDF |
43.6ab |
43.5a |
43.3a |
48.0ab |
48.3b |
1.09/0.013 |
ADF |
22.7a |
24.1ab |
25.5b |
29.3c |
30.8c |
0.52/0.001 |
Means with different letters within the same row are different at the 5% level. |
|
Figure 1. Relative intakes of Mucuna and Para grass according to offer level of Mucuna in Experiment 1 |
Table 3: Projected and actual ratios of Mucuna and para grass that were consumed |
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Planned |
0 |
0.20 |
0.40 |
0.60 |
0.80 |
Observed |
0.00 |
0.20 |
0.38 |
0.48 |
0.60 |
Growth rate increased with increasing ratio of Mucuna as replacement for Para grass until a substitution level of about 0.50 (Table 4; Figure 2) after which it appeared to decrease. Feed conversion became worse as the proportion of Mucuna increased beyond 0.2 (Figure 3).
Table 4. Mean values for daily weight gain and feed conversion in growing rabbits fed different ratios of Mucuna and Para grass in Experiment 1 |
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|
Observed ratio Mucuna to Para grass (DM basis) |
|
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|
0.00 |
0.20 |
0.38 |
0.48 |
0.60 |
SEM/Prob. |
Live weight, g |
|
|
|
|||
Initial |
614 |
614 |
614 |
615 |
617 |
2.65/0.842 |
Final |
2059a |
2113a |
2126a |
2253b |
2153ab |
25.6/0.004 |
Daily gain |
18.8a |
19.5a |
19.6a |
21.3b |
19.9ab |
0.33/0.004 |
Feed conversion ratio |
4.07a |
4.07a |
4.27a |
4.47a |
5.40b |
0.13/0.001 |
Means with different letters within the same rows are different at the 5% level. |
Table 5. Chemical composition of feeds (% in DM, except for DM which is on fresh basis) used in Experiment 2 |
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|
DM |
OM |
CP |
NDF |
ADF |
Ash |
Mucuna pruriens |
19.8 |
90.6 |
14.7 |
42.7 |
38.3 |
9.40 |
Para grass |
18.6 |
89.8 |
9.10 |
71.8 |
35.8 |
10.2 |
Soybean waste |
11.1 |
93.2 |
21.7 |
47.9 |
33.4 |
6.80 |
Concentrate |
87.0 |
91.1 |
20.0 |
23.6 |
8.06 |
8.90 |
DM feed intake tended to show a similar pattern to what was observed in Experiment 1, with a progressive increase until the ratio of Mucuna to Para grass reached 0.5. However, in contrast to what was observed in Experiment 1, intake appeared to decrease when the offer level of Mucuna exceeded 0.5 of the forage fraction (Table 6; Figure 4).
Table 6. Daily intakes of forages and proximate constituents in Experiment 2 (g/rabbit/day) |
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Items |
Treatments |
SEM/Prob. |
||||
MP0 |
MP20 |
MP40 |
MP60 |
MP80 |
||
DM |
72.3 |
74.6 |
74.4 |
80.5 |
73.7 |
1.96/0.102 |
OM |
67.3 |
68.6 |
68.7 |
74.1 |
67.5 |
1.79/0.118 |
CP |
11.4a |
12.4ab |
12.5ab |
13.6b |
13.4b |
0.29/0.002 |
NDF |
37.1a |
36.6ab |
34.0ab |
36.5ab |
30.4b |
1.40/0.036 |
ADF |
19.5a |
20.9ab |
21.1ab |
23.5b |
20.8ab |
0.72/0.004 |
Means with different letters within the same rows are different at the 5% level. |
|
Figure 4. Relative intakes of Mucuna and Para grass according to offer level of Mucuna in Experiment 2 |
Table 7. Apparent digestibility coefficients and N balance for rabbits in Experiment 2 |
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|
Observed ratio Mucuna to Para grass (DM basis) |
SEM/Prob. |
||||
|
|
|
|
|
||
Apparent digestibility, % |
|
|
|
|
|
|
DM |
61.1 |
65.5 |
67.1 |
67.6 |
66.8 |
1.59/0.080 |
OM |
61.5 |
65.5 |
67.3 |
67.6 |
67.1 |
1.60/0.105 |
CP |
81.2 |
83.1 |
81.4 |
81.2 |
80.6 |
1.24/0.678 |
NDF |
42.0a |
49.9ab |
50.3ab |
51.6b |
47.9ab |
2.07/0.050 |
ADF |
32.6a |
43.8b |
48.0b |
48.8b |
45.6b |
2.23/0.010 |
N balance, g/kg LW/day |
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Intake |
1.25 |
1.36 |
1.36 |
1.43 |
1.51 |
0.04/0.002 |
Retention |
0.71 |
0.79 |
0.79 |
0.81 |
0.94 |
0.05/0.057 |
Means with different letters within the same rows are different at the 5% level. |
The ratio of Mucuna to Para Grass did not affect the apparent digestibility coefficients of DM, OM and CP (Table 7); however, the coefficients for the NDF and ADF fractions followed the same trend as for intake with increases up to 0.50 Mucuna followed by a decline at the higher level (Figures 5 and 6).
Intake and retention of N increased with increasing ratios of Mucuna (Table 7; Figures 7 and 8). Probably the higher intakes of digestible crude protein and the higher digestibility of the cell wall fractions in the Mucuna contributed to the improvement in N retention which, except at the highest Mucuna ratio, mirrored effects of the Mucuna ratio on growth rate.
The authors would like to thank the Department of Animal Science, Faculty of Agriculture and Applied Biology of Cantho University.
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