Proceedings Buffalo Workshop December  2001

http://www.mekarn.org/procbuf/phuo.htm

Study on the application of molecular biological techniques in typing of Pasteurella multocida in buffalo and cattle
 

Phan Thanh Phuong, Truong Van Dung, Dinh Duy Khang,
Hoang Xuan Nghinh, La Van Phan

 

Abstract

PCR technique with two specific primer pairs HB₁, HB₂ and HS₁, HS₂ was used for typing Pasteurella multocida. Among 7 national reference strains (4I, I₂, IR 43, 99, 483, 8936) only strain IR (type B Iran) showed specific PCR pattern as type B.

 

Most of isolated strains of Pasteurella multocida from Central and Northern part of Vietnam (27/28) have specific PCR pattern as type B.

 

The technique with specific primer pair TOX₁, TOX₂ was also used for detection of toxigenic P.multocida. From 35 tested strains (7 national reference strains and 28 isolated strains) only strain 99 showed specific PCR pattern as toxigenic P. multocida.

 

The Western blot technique was applied for determining  of P. multocida strains as IR, 99 and 483.