The experiment was conducted at An Giang University, Viet Nam where the annual temperature during the experiment ranged from 27 to 38°C. The aim of the study was to determine the effect of method of processing cassava leaves on the intake, apparent digestibility and nitrogen retention and HCN content by indigenous piglets of diets based on broken rice. Four indigenous piglets of 8.3 to 9.7 kg and 3 months of age were used in the experiment. They were housed in individual bamboo cages and allocated to two treatments according to a single changeover design. The treatments were cassava leaves given ad libitum in fresh or wilted form as supplements to a basal diet of broken rice fed at the rate of 2% (DM) of live weight.
The actual intakes of cassava leaves and total DM were similar
between the two treatments
(P>0.05). Total dry matter intake was rater low in
the range from 2.6 to 2.8% of body weight. The cassava leaves represented
from 24 to 27.4 % of the total diet dry matter, providing about 50% of the total
dietary protein. HCN levels were reduced from by wilting the cassava leaves
(from 269 to 42 mg/kg DM). Average values for DM, OM and N digestibility were 89.1, 89.7 and 73.9%
for the diet with fresh cassava leaves, and for the diet with wilted
cassava leaves, 90.9, 91.7 and 76.6%, and did not differ between
treatments
(P>0.05).
Based on the results of this research it was concluded that fresh cassava leaves can safely be fed to growing pigs at levels up to about 25% of the diet. The HCN content in the fresh leaves did not appear to be a constraint as there was no advantage in wilting the leaves which reduced by six-fold the level of HCN.
In view of the predicted world shortage of cereal grains because of competing needs for the expanding human and livestock populations (Leng 2002), there is an urgent need for research to develop alternative feed resources especially for pigs and poultry.
Cassava (Manihot esculenta Crantz) is a widely grown crop in the tropical regions of Africa, Latin American and Asia (Calpe 1993). The leaves are high in protein (Ravindran and Ravindran 1988) and are a readily available product at the time of harvesting the root. Several reports have shown that cassava leaf protein is rich in lysine but low in sulphur amino acids (Eggum 1970; Gomez and Valdivieso 1984).
In Vietnam cassava is an important food crop, and annual root production is about 2 millions tonnes (GSO 2001) the majority of which is used for animal feeding and production of starch. Cassava leaf is a rich source of protein, minerals and vitamins, with an average of 21% crude protein (range from 16.7 to 39.9%) (Eggum 1970; Allen 1984; Bui Van Chinh et al 1994).
In fresh cassava leaf there is a high content of glucosides, linamarin and
lotaustralin, which are hydrolysed by the linamarase enzyme, resulting in the
release of hydrocyanic acid (HCN), which is toxic to the animal. (Hoang van Tien 1987).
HCN is colourless, volatile and extremely poisonous. The content
of HCN in cassava differs among varieties and generally ranges from 200 to 800 mg /kg
DM in the fresh leaf but values as low as
80 mg/kg DM (Wood 1965) and as high as over 4000 mg/kg DM
(Ravindran and Ravindran 1988) have been reported. The HCN content also depends on
the nutritional
status of the plant, and is increased by N fertilization (De Bruilin
1973). The glucoside concentration in cassava leaves decreases with age (Lutaladio 1984; Ravindran and Ravindran 1988). The
elimination of cyanogens by heating will depend on the temperature, the stage of development of
the plant, and the type of heat. Simple
sun drying or oven drying has been reported to eliminate almost 90%
(Oke 1994) and sun drying reduces the cyanogen content of cassava
leaf more effectively than ensiling because of the stability of the
linamarase at low pH values (Oke 1994). Despite its high content of
HCN, documented cases of poisoning due to the ingestion of cassava
leaf are rare (Ravindran 1993).
Based on this theoretical and practical background, an experiment was carried out to evaluate the effects of method of processing cassava leaves on intake, digestibility, N retention by pigs and on the HCN content in cassava leaves in the fresh and wilted form. The aims of using cassava leaves as a supplementary source of protein in animal diets is as a means of reducing the cost of feed and increasing the income for the farmer.
The experiments were carried out in An Giang University -An Giang province, Vietnam from 5 August, 2003 to 25 August, 2003. The annual temperature in this location is in the range of 27 to 38°C.
The experimental design was a single changeover arrangement (Table 1) with two periods and 4 replicates.
Table 1. The experimental layout |
||||
Periods/Pigs |
Pig 1 |
Pig 2 |
Pig 3 |
Pig 4 |
1 |
FC |
WC |
FC |
WC |
2 |
WC |
FC |
WC |
FC |
The experimental period was 10 days: five days for adaptation to allow the pigs to become familiarized with the new diet and a five days period for collection of faeces, urine and feed residue.
Four local male pigs (Ba Xuyen breed) with live weight of 8.3 to 9.7 kg and age of 3 months were used. The pigs were housed individually in metabolism cages made from bamboos strips fixed to a wooden frame in a composite unit (1.8m length and 0.8m wide) for 2 animals per unit (Photo 1). The metabolism cages allowed the pigs to move freely. The cages were fitted with automatic water drinkers. Plastic netting was suspended below the floor to collect the faeces. The urine passed through the plastic net and was collected over a sheet of polyethylene leading to a filter placed in a funnel suspended over a plastic bucket containing sulphuric acid (10 ml of 10% sulphuric acid) so as to maintain the pH below 4.0.
Photo 1: The metabolism cage made from bamboo for
measuring digestibility and N retention
Broken rice was fed at the rate of 2% (DM basis) of live weight. The cassava leaves were offered on a free choice basis. Drinking water was available via automatic valves.
During the 5-day collection period, the faeces were collected every morning before feeding and kept in polyethylene bags at -20 oC. The total volume of urine was recorded daily and 10% of the total collection retained until the end of each period, when a representative sample was taken for analysis. At the end of the collection period the faeces were thawed and mixed thoroughly to provide a representative sample for each pig. The pigs were weighed at the beginning of the experiment and at the end of each period. The feed offered and refused was weighed and recorded daily and a sample was kept for analysis.
Feeding behaviour of the pigs was recorded by direct observation every minute during 90 minutes following feeding, according to the method of Faliu and Griess (1968).
Dry matter and nitrogen were determined in feed offered and refused and in faeces. The urine was analysed for N. The chemical analyses were done following standard procedures according to the Association of Official Analytical Chemists (AOAC 1988), except for DM which was determined by micro-wave radiation (Undersander et al 1993). Water extractable DM was assayed as outlined by Ly and Preston (1997). Total volatile fatty acids in faeces were determined by steam distillation and titration of the distillate with NaOH 0.01N.
The data for feed intake, apparent digestibility and N balance
were subjected to analysis of variance according to the General
linear model of the Minitab software (version 13.3)
The composition of the feeds is shown in Table 2. The value for nitrogen content of cassava leaves indicates that the protein level is about 23% which is line with the average values in the literature of around 21% crude protein (Eggum 1970; Allen 1984; Bui Van Chinh et al 1994). The low nitrogen content of the broken rice shows that the protein content is about 8% in DM and is therefore mainly an energy source. The values for HCN in cassava leaves (41.7 mg/kg DM in wilted compared with 269 mg/kg DM in fresh leaves, shows that HCN content was considerably reduced when the leaves were wilted. These levels are considerably lower than those reported by Ravindran (1991) who quoted a range of 800 to 3,200mg HCN/kg DM of cassava leaves.
Table 2: Chemical characteristics of the ingredients of the diets (% dry basis except for DM which is on fresh basis; HCN is in mg/kg DM) |
|||
|
Fresh cassava |
Wilted cassava |
Broken rice |
Dry matter |
26.2 |
60.5 |
85.0 |
Ash |
15.4 |
12.2 |
0.78 |
Organic matter |
84.6 |
87.8 |
99.2 |
N |
3.72 |
3.70 |
1.4 |
HCN |
269 |
41.7 |
- |
Water extractable DM |
43.7 |
25.9 |
- |
Water extractable N |
26.4 |
30.0 |
- |
The data in Table 3 show that the actual intakes of cassava leaves and total DM were similar between treatments with an indication that cassava leaf intake (P=0.080) and DM intake as percentage of body weight (P=0.087) were increased slightly by wilting. Total dry matter intake was rather low in the range of 2.6 to 2.8% body weight. With controlled offer of broken rice at 2% of body weight (DM basis) and free access to cassava leaves, the intake of the latter accounted for 24 to 27.4 % of the total diet dry matter, and provided from 44 to 48% of the total protein.
Table 3: Mean value for feed intake of pigs fed cassava leaves and broken rice |
||||
|
Fresh cassava |
Wilted cassava |
SEM |
Prob. |
Intake, g DM/day |
|
|
|
|
Broken rice |
182 |
183 |
6.87 |
0.930 |
Cassava leaves |
58.5 |
75.8 |
5.80 |
0.080 |
Total DM |
240 |
259 |
9.57 |
0.229 |
Cassava as % of total DM |
23.8 |
28 |
2.01 |
0.198 |
DM, % BW |
2.6 |
2.8 |
0.06 |
0.087 |
Crude protein |
|
|
|
|
Total, g/day |
30 |
34 |
1.55 |
0.119 |
As % DM |
12.4 |
12.9 |
0.27 |
0.215 |
Digestibility coefficients for DM, organic matter and nitrogen were high (Table 4; Figure 1) and did not differ between treatments.
Table 4: Digestibility coefficients for pigs fed broken rice supplemented with fresh or wilted cassava leaves |
||||
|
Fresh cassava |
Wilted cassava |
SEM |
Prob. |
DM |
89.1 |
90.9 |
1.75 |
0.50 |
OM |
89.7 |
91.7 |
1.52 |
0.40 |
N |
73.9 |
76.6 |
5.00 |
0.72 |
Figure 1: Digestibility
coefficients of dry matter, organic
matter and nitrogen for pigs
fed broken rice and fresh or wilted cassava leaves.
There was also no treatment effect (P>0.05) on N retention (Table 5). However, N retention as percent of N intake and N digested were rather low.
Table 5: N retention of piglets fed broken rice and fresh or wilted cassava leaves |
||||
|
Fresh cassava |
Wilted cassava |
SEM |
Prob. |
N balance, g/d |
|
|
|
|
Intake |
4.80 |
5.43 |
0.25 |
0.122 |
Faeces |
1.26 |
1.16 |
0.23 |
0.755 |
Urine |
1.61 |
2.12 |
0.51 |
0.504 |
Digested |
3.53 |
4.28 |
0.39 |
0.226 |
Retention |
1.93 |
2.16 |
0.59 |
0.789 |
Retention as % of |
|
|
|
|
Digested N |
39.4 |
35.9 |
9.27 |
0.798 |
Intake of N |
51.8 |
43.6 |
10.75 |
0.612 |
Figure 2: Effect of N intake on N
retention
N retention increased linearly as N intake increased (Figure 2), suggesting that the low DM intake was the major constraint limiting N retention.
Feeding behaviour was observed and recorded (Table 6
Table 6: Feeding behaviour of pigs fed broken rice and cassava leaves |
||||
|
Fresh leaves |
Wilted leaves |
SEM |
Prob. |
Time eating, min |
56.5 |
54.8 |
6.45 |
0.854 |
Frequency of eating, times |
8.00 |
8.00 |
1.85 |
1.00 |
Frequency of eating, min |
7.25 |
7.97 |
1.60 |
0.76 |
Fresh cassava leaves were consumed by growing pigs at levels equivalent to
almost 30% of the DM intake and there appeared to be no benefit from wilting the
leaves, even although this reduced six-fold the HCN concentration, Thus, according to the present experiment,
the cyanogenic glucosides present in cassava leaves are not the major factor
governing their utilization by growing pigs. Nevertheless DM intakes
expressed as percentage of body weight were less than 3%, which is below the
expected norm for pigs of 10 kg body weight which should be between 4 and 5%.
Further research is needed to determine the factors that limit feed intake when
the aim is to use fresh cassava leaves as the major protein supplement for
growing pigs.
The mini-project was carried out at An Giang University – Vietnam. I would like to thank the SIDA- SAREC for funding this mini-project – a part of the MSc program course through the regional MEKARN project. I would like to express gratitude to Dr Thomas R Preston, Dr Julio Ly and Dr Do van Xe for patient guidance and encouragement and make it possible for us to complete the mini-project. And I also would like to express my sincere thanks to staffs of UTA and An Giang University who provided valuable assistance in helping to analyze the data in the laboratory.
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Mahendranathan T 1971 The effect of feeding tapioca (Manihot utilissima Pohl) leaves to pigs Malays.Agric. J. 48: 60-68. Quoted by Bui Huy Nhu Phuc, Ogle B and Lindberg J E 2003 from International Workshop Current Research and Development on use of Cassava as Animal Feed. Khon Kaen University, Thailand. July23-24, 2001 http://www.mekarn.or/procKK/phuc.htm
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