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Determination of pH
The
pH value in different types of samples is determined by a portable digital
pHmeter. The principle of the determination is based on potentiometry. Thee
pHmeter has a pH combination electrode and pH values can be estimated in the
range of 0.00 to 14.00 with a resolution of 0.01 and accuracy of 0.01.
Instructions
1/
Connect the pHmeter with the pH electrode and switch. Set the mode switch to thr
pH
position. This will activate the liquid cristal display. Condition the
electrode in buffer 7.00
until the display is fully stable (about 30 minutes). Give a stirring
action to the electrode each time it is placed in a new solution.
2/
Adjust the calibration control to make the display read the value of buffer
7.00. Rinse the electrode with distilled water and blot dry.
3/
Inmerse the electrode in buffer 4.00. When the display is stable (30 seconds),
adjust the slope control to make the display read the value of buffer 4.00. The pH
value are 6.99 and 4.01 for buffer 7.00 and 4.00 respectively at 30oC. Error may be minimum
in the case that
pH value are not adjust to the temperature of the solution to be tested.
The apparatus is now calibration to read samples with pH 0 - 14.
4/
Rinse the electrode with distill water, blot dry and inmerse in the saple. The
sample must
be homogenous. When the display is stable (30 seconds), it will read the
pH of the sample
Repeat this step for continued samples. Periodically check calibration,
at least one per day.
5/
When all samples are completed, keep the electrode inmerse in distilled water.
The
electrode shall never be kept dry, otherwise electrode damage shall be
irreversible.
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