Bottle
2 : 93.05g EDTA + 34.05g sodium tetraborate + 1 litter water
Bottle
3 : 22.8g Disodium hydrogen phosphate + 1 litter water gently boiling and cool
down
Bottle
4 = put 2 and 3 into 1 adiust until 5 litters and correct pH 6.9 - 7.0 it OK
Weigh
accurate 1 - 1.5g of the grinded sample into a beaker (600ml) or flask. Add 100
ml of NDF - solution and 2 ml of decalin.
Heat
to boil and reflux for 60 minute
Place
a previously tared glassfillter - crucible on the filtermaniflold. Transfer
contents of beaker to the crucible while using low vacuum from the beginning.
Rinse beaker with hot water and also the residue in the crucible with hit water.
Rinse finally twice with acetone. Dry crucible at 105oC over night. Weigh out
after cooling down in a desiccator. Ash at 500oC for 2 - 3 hours. Cool down in
desiccator and weigh again.
OS - DR
% WL (DM) = -------------- x
100
OS
Weigh
after drying - Tare weight
NDF (INCL ASH) % = ------------------------------------ x 100
Sample weight
Weigh
after drying - weigh after ASH
NDF (EXCL ASH) % = --------------------------------------- x 100
Sample weight